反应详情

The slides produced with cells grown in presence of either Cd+Cr+H2O2 or 5-azacytidine, or sodium butyrate are incubated overnight at 4° C. in the presence of 30 μl of an anti-5 methylcytosine sheep antibody (Abcam, Ab1884) diluted 1/100 in PBS. The slides are washed once in PBS and then incubated for 15 seconds in the presence of 30 μl of NaOH 7×10−5 N. After washing with PBS, the slides are finally incubated 30 minutes in the presence of a donkey anti-sheep IgG antibody (southern Biotechnology Associates, Inc) diluted at a concentration of 0.1 μg 1 1×106 cells in PBS.

1. customThe slides produced with cells
2. additiondiluted 1/100 in PBS
3. washThe slides are washed once in PBS
4. washAfter washing with PBS
5. waitthe slides are finally incubated 30 minutes in the presence of a donkey anti-sheep IgG antibody (southern Biotechnology Associates, Inc)
6. additiondiluted at a concentration of 0.1 μg 1 1×106 cells in PBS