Azacytidine → HCID 178430896, compound

HRID 1367451Notes

Reaction equation

HRID 1367451 reaction equation
[C@@H:1]1([N:10]2C=[N:16][C:14](N)=[N:13][C:11]2=[O:12])O[C@H](CO)[C@@H:4](O)[C@H:2]1O.C([O-])(=O)CCC.[Na+]>>[CH3:4][C:2]1[C:14]([NH2:16])=[N:13][C:11](=[O:12])[NH:10][CH:1]=1

Participants

3

Reactant 2

HCID 9444AzacytidineHCID 5222465Sodium Butyrate

Product 1

HCID 178430896HCID 178430896, compound

Procedure

The slides produced with cells grown in presence of either Cd+Cr+H2O2 or 5-azacytidine, or sodium butyrate are incubated overnight at 4° C. in the presence of 30 μl of an anti-5 methylcytosine sheep antibody (Abcam, Ab1884) diluted 1/100 in PBS. The slides are washed once in PBS and then incubated for 15 seconds in the presence of 30 μl of NaOH 7×10−5 N. After washing with PBS, the slides are finally incubated 30 minutes in the presence of a donkey anti-sheep IgG antibody (southern Biotechnology Associates, Inc) diluted at a concentration of 0.1 μg 1 1×106 cells in PBS.

Workup

  1. customThe slides produced with cells
  2. additiondiluted 1/100 in PBS
  3. washThe slides are washed once in PBS
  4. washAfter washing with PBS
  5. waitthe slides are finally incubated 30 minutes in the presence of a donkey anti-sheep IgG antibody (southern Biotechnology Associates, Inc)
  6. additiondiluted at a concentration of 0.1 μg 1 1×106 cells in PBS

Sources & evidence

ORD record
ord-5f68a6a89d8048faa0c8a0cde51d06e6
Source dataset
uspto-grants-2011_02
Patent number
US07893250B2

Reaction notes

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