Acetic Acid → FR-179642

Participants

Role	Name	HCID	Charge
Reactant	Acetic Acid	HCID 176	—
Reactant	[5-[(1S,2S)-2-[(3S,6S,9S,11R,15S,18S,20R,21S,24S,25S,26S)-3-[(1R)-3-amino-1-hydroxy-3-oxopropyl]-18-(hexadecanoylamino)-11,20,21,25-tetrahydroxy-15-[(1R)-1-hydroxyethyl]-26-methyl-2,5,8,14,17,23-hexaoxo-1,4,7,13,16,22-hexazatricyclo[22.3.0.09,13]heptacosan-6-yl]-1,2-dihydroxyethyl]-2-hydroxyphenyl] hydrogen sulfate	HCID 44352613	—
Reactant	Disodium hydrogenphosphate potassium dihydrogenphosphate	HCID 71312623	—
Solvent	Methanol	HCID 887	—
Product	FR-179642	HCID 71714631	—

Reaction Steps

To 700 μl of the fermentation broth of Streptomyces sp. No. 6907 obtained in Example 1-3 was added 100 μl of an aqueous solution of Substance FR901379 (100 mg/ml) (10 mg as Substance FR901379; 8.35 μmol) together with 100 μl of methanol and 100 μl of buffer (0.5 M potassium dihydrogen phosphate-disodium hydrogen phosphate buffer; pH 6.0), and the reaction was carried out at 30° C. for 30 minutes. The reaction was then stopped with 4% acetic acid and following addition of 2 ml of methanol, the mixture was filtered through a membrane filter (0.45 μm) to remove the macromolecular protein and other fraction and subjected to HPLC and the acylase activity of the skeletal substance FR179642 produced was monitored and assayed at 210 nm.

Provenance & Evidence

DOI	10.6084/m9.figshare.5104873.v1
Patent number	US06573084B1
Source dataset	uspto-grants-2003_06
ORD ID	ord-505864764e014c72a4efb55e3aab965b
Source note	uspto-grants-2003_06