Acetic Anhydride → HCID 16122493, compound

Participants

Reaction Steps

3,19-Isopropylidene andrographolide (15 g) obtained in step 1 was refluxed in distilled acetic anhydride (110 ml) for 45 min. After confirming the complete formation of the product (by TLC analysis), the contents were cooled to room temperature, diluted with water (500 ml) and extracted with dichloromethane (3×200 ml). The organic layer was separated and dried over Na2SO4 and concentrated to get a brown oily material. The crude material was purified by flash column chromatography (silica gel 230-400 mesh; 250 g, eluting system light petrol: ethyl acetate=85:15) to obtain pure 14-acetyl-3,19-isopropylidene andrographolide (13 g).

Provenance & Evidence