反应详情

A solution of (3S)-3-[N-((1-naphthyloxy)acetyl)leucinyl]amino-4-oxobutanoic acid semicarbazone (0.320 g, 0.68 mmol) in 37% aqueous formaldehyde(1.0 mL)-acetic acid(1.0 mL)-methanol(3.0 mL) was stirred at room temperature under nitrogen for 3.5 hrs. The resulting solution was diluted with water and extracted with EtOAc. The extract was washed with water and saturated NaCl solution, dried over anhydrous Na2SO4 and evaporated to dryness. The residue was taken up in EtOAc, filtered through Celite and evaporated to dryness. The product was taken up in a small amount of dioxane, diluted with water, frozen and lyophilized to give the title compound (0.222 g, 79%) as a fluffy white solid. TLC(EtOAc-pyridine-AcOH-H2O; 60:20:5:10) Rf=0.65.

1. extractionextracted with EtOAc
2. washThe extract was washed with water and saturated NaCl solution
3. dry with materialdried over anhydrous Na2SO4
4. customevaporated to dryness
5. filtrationfiltered through Celite
6. customevaporated to dryness
7. additiondiluted with water
8. temperaturefrozen