Sec-Butylamine → (-)-Norfenfluramine

Participants

Reaction Steps

The Arthrobacter species KNK168 strain was subjected to shaking culture by using (RS)-1-methylpropylamine as an inducer for enzyme in the same manner as Example 6. The cells harvested from 1 liter culture were suspended in 1 liter of 0.1 M Tris-hydrochloric acid buffer. Thereto were added a mixture of 30 g 1-(3-trifluoromethylphenyl)-2-propanone, 18 g (R)-1-phenylethylamine, and 44 g oleic acid (pH 8.5), and the resulting mixture was reacted at 30° C. for 40 hours. After reaction, the extraction and distillation were carried out in the same manner as in Example 6, to give 19.5 g (R)-1-(3-trifluoromethylphenyl)-2-aminopropane. The optical purity was 100% ee, and the yield was 65%.

Provenance & Evidence