Thymidine → HCID 67703941, compound

Participants

Reaction Conditions

Reaction Steps

6-Propylthio-9H-purine (0.50 g, 2.6 mmoles) (Alfred Bader Division of Aldrich Chemical Company) and 2',3'-dideoxy-3'-fluorouridine (0.50 g, 2.2 mmoles) were suspended in 500 ml 10 mM potassium phosphate buffer, pH 7.0, containing 0.04% potassium azide. Purine nucleoside phosphorylase (1120 I.U.) and thymidine phosphorylase (10,000 I.U.) (Krenitsky, et al., Biochemistry, 20, 3615, 1981 and U.S. Pat. No. 4,381,344) immobilized on DEAE cellulose was added to the reaction and the suspension was stirred at 45° C. After 4 days, 175 ml MeOH was added to the reaction. The reaction was applied to a column containing AG1-X2 (OH-- form, 2.5×10 cm) and eluted with MeOH. Fractions containing product were pooled, concentrated, and flash chromatographed on silica gel (2.5×20 cm) with dichloromethane:acetone (9:1). The product was then further chromatographed on silica gel (2.5×20 cm) with dichloromethane:acetone (98:2). Solvent was removed and lyophilization yielded 0.27 g of 9-(2,3-dideoxy-3-fluoro-β-D-erythro-pentofuranosyl)-6-propylthio-9H-purine (39%): mp 85°-86° C.; TLC Rf 0.87 (silica gel, MeCN:15N NH4OH:H2O/85:5:10); [α]D20 -20.2° (c=0.5, DMF): UV λmax (ε×103-) at pH 7, 293 (20.0); at pH 13, 292 (20.4); 1H NMR (300 MHz, DMSO-d6) δ8.74 and 8.68 (2s, 2H, H2 and H8), 6.48 (dd, 1H, H1', J=9.0 Hz, J=5.8 Hz), 5.46 (dd, 1H, H3', J=53.4 Hz, J=4.2 Hz), 5.21 (t, 1H, OH5', J=5.6 Hz), 4.26 (dt, 1H, H4', J=26.2 Hz, J=4.8 Hz), 3.59-3.62 (m, 2H, H5' and H5"), 3.25 (b, 2H, SCH2CH2CH3), 2.95-3.20 and 2.62-2.80 (2m, 2H, H2' and H2"), 1.69-1.76 (m, 2H, SCH2CH2CH3) and 1.00 (t, 3H, SCH2CH2CH3, J=7.4 Hz); MS (ci) 313 (M+1), 293 (M-F), 195 (MH2 --C5H8FO2).

Provenance & Evidence