Hydroxyamine hydrochloride → HCID 131720180, compound

Participants

Reaction Steps

Under an inert atmosphere, 142 mg of (1,1-dimethyl)ethyl 7-[2-(2-tritylamino-thiazol-4-yl)-2-methoxyimino-acetamido]-3-formyl-8-oxo-4-thia-1-azabicyclo[4,2,0]oct-2-en-2-carboxylate, (prepared in Step A of Example 52) and 42 mg of hydroxylamine hydrochloride were dissolved in 0.4 ml of pyridine and the solution was stirred for 50 minutes and diluted with ether and with ethyl acetate. The mixture was washed with 6 ml of N hydrochloric acid and after extracting the aqueous phase with ethyl acetate, the organic phases were dried and concentrated to dryness. The residue was chromatographed under pressure on silica and eluted with a mixture of ethyl acetate and methylene chloride (25-75) to obtain 91 mg of 1,1-dimethy]-ethyl 7-[2-(2-tritylamino-thiazol-4-yl)-2-methoxyimino-acetamido]-3-hydroxyiminomethyl-8-oxo-4-thia-1-azabicyclo[4,2,0]oct-2-en-2-carboxylate.

Provenance & Evidence