Trifluoroacetic acid → N-[(Benzyloxy)carbonyl]-L-phenylalanyl-L-leucinamide

Participants

Reaction Steps

30 mg (0.1 mole) Z-Phe-OH and 13 mg (0.1 mmole) Leu-NH2 are placed into an unsealed glass container having a 12 mm inner diameter and stirred with a spatula. Then, a mixture of 161 mg (0.5 mole) Na2 SO4.10H2O and 5 mg thermolysine is added, the glass container is closed and subjected to ultrasonic waves in an ultrasound bath at 40° C. After 20 minutes the reaction is stopped with 0.4 ml 5% (v/v) aqueous trifluoroacetic acid. The analytical evaluation is carried out by means of HPLC and comparison with an authentic specimen.

Provenance & Evidence