反应详情

温度

50 °C

Reaction--Ten grams of the enzyme adsorbed on celite were added to 300 ml of 1% rifamycin B solution (10 mM phosphate buffer, pH 8.5). The conversion to rifamycin S was completed in 7 hours of reaction at 40° C. with aeration and agitation. The celite filter cake recovered by filtration on a sintered glass was washed with 200 ml of methanol. The final methanolic aqueous solution of rifamycin S was warmed to 50° C. in order to solubilize completely the residual rifamycin S precipitate. 30 ml of 5% ascorbic acid solution in water was added thereto dropwisely while continuous stirring. The purpose color of rifamycin S was gradually changed to the deep red color of rifamycin SV. After adjusting the final pH to 4.0, the methanol was completely evaporated using suction evaporator at 40° C. Afte chilling the final solution at 4° C. for 2 days, the rifamycin SV crystals were recovered by filtration. 2.4 gr of deep yellowish rifamycin SV crystals were obtained after drying over sodium sulfate (yield=87%).

1. customReaction
2. filtrationThe celite filter cake
3. filtrationrecovered by filtration on a sintered glass
4. washwas washed with 200 ml of methanol
5. addition30 ml of 5% ascorbic acid solution in water was added
6. customthe methanol was completely evaporated
7. customsuction evaporator at 40° C
8. temperatureAfte chilling the final solution at 4° C. for 2 days
9. filtrationthe rifamycin SV crystals were recovered by filtration