反应详情

温度

37 °C

[L-arginine deiminase activity is indicated in terms of micromoles of L-citrullin which are produced by reaction with L-arginine. The reaction with L-arginine is conducted by adding 10 g of the immobilized preparation to an aqueous 0.5 M L-arginine hydrochloride solution (pH 6.0) containing 0.01% of triethanolamine laurylsulfate, and shaking the mixture at 37° C. for one hour. The amount of L-citrullin produced is assayed colorimetrically by using diacetylmonooxim as a coloring agent.]

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