Magnesium Chloride → L-Aspartic Acid

HRID 1390198Notes

Reaction equation

HRID 1390198 reaction equation
[C:1]([O-:8])(=[O:7])/[CH:2]=[CH:3]/[C:4]([O-:6])=[O:5].[NH4+:9].[NH4+].[Cl-].[Mg+2].[Cl-]>>[NH2:9][C@H:2]([C:1]([OH:8])=[O:7])[CH2:3][C:4]([OH:6])=[O:5]

Participants

3

Reactant 2

HCID 5360315Magnesium ChlorideHCID 9793847Ammonium fumarate

Product 1

HCID 5960L-Aspartic Acid

Procedure

60.4 g of the immobilized Escherichia coli preparation obtained in paragraph (1) are packed in a column (4 cm in diameter and 8 cm in height), and incubated at 37° C. for 48 hours. After incubation, the immobilized preparation shows an aspartase activity of 278, 422μ moles/hr. Then, 1000 ml of an aqueous 1 M ammonium fumarate solution (pH 8.5) containing 1 mM magnesium chloride are passed through the column at 37° C. at a flow rate of 50 ml/hr. The effluent is adjusted to pH 2.8, and the crystalline precipitates are collected by filtration. 122 g of L-aspartic acid are obtained.

Workup

  1. custom60.4 g of the immobilized Escherichia coli preparation
  2. customAfter incubation, the immobilized preparation
  3. customare passed through the column at 37° C. at a flow rate of 50 ml/hr
  4. customthe crystalline precipitates
  5. filtrationare collected by filtration

Sources & evidence

ORD record
ord-78c0b4ee9fbf4e00af038bc8939cb2be
Source dataset
uspto-grants-1979_02
Patent number
US04138292

Reaction notes

Loading…