反应详情

Batch fermentations were prepared to determine the overall production of flavonoid compounds using commercially available (2S)-eriodictyol. An overnight preinnoculums of recombinant E. coli BL21Star (Invitrogen) was prepared carrying vectors pCDF-F and pCOLA-DL in LB or M9 minimal media. Antibiotics were added according to manufacturer's instructions (50 ug/mL streptomycin and 30 ug/mL kanamycin). The next day, 4 mL of the preinnoculum was used to seed 76 mL LB or M9 minimal media cultures. The culture was grown in an incubator at 37° C. until OD600 reaches about 0.6. Gene expression was induced by 1 mM ITPG and the cultures were grown at 27° C. with horizontal shaking for 4 hours. (2S)-Eriodictyol dissolved in DMSO was added to a final concentration of 0.2 mM. The cultures were incubated at 27° C. for 72 hours.

1. customBatch fermentations were prepared
2. customAn overnight
3. customcoli BL21Star (Invitrogen) was prepared
4. additionAntibiotics were added
5. customwas grown in an incubator at 37° C. until OD600 reaches about 0.6
6. customwere grown at 27° C.
7. additionwas added to a final concentration of 0.2 mM
8. waitThe cultures were incubated at 27° C. for 72 hours