反应详情

Compound 3 (200 mg, 0.53 mmol) was dissolved in methanol (7.00 ml), and 28% aqueous ammonia (5.00 ml) was added to the resultant solution, followed by stirring at room temperature for four hours. The resultant reaction mixture was concentrated under reduced pressure, and a mixture of chloroform and methanol (20:1) was added to the resultant residue. The thus-precipitated crystals were filtered, and then the resultant crystals were recrystallized from water, to thereby yield compound 4 (113 mg, 0.39 mmol, 73.6%).

1. customThe resultant reaction mixture
2. concentrationwas concentrated under reduced pressure
3. additiona mixture of chloroform and methanol (20:1)
4. additionwas added to the resultant residue
5. filtrationThe thus-precipitated crystals were filtered
6. customthe resultant crystals were recrystallized from water