Ammonium fluoride → HCID 58661645, compound

To a stirred solution of crude 2-[2-(3-methylphenyl)ethoxy]-2′,3′,5′-tri-O-(TERT-butyldimethylsilyl)-adenosine (5 g, Example 8) in anhydrous methanol (70 mL) under a nitrogen atmosphere was added ammonium fluoride (2.5 g, 67.5 mmol). The resulting solution was heated to reflux for 14 hours, at which time HPLC indicated the disappearance of starting material. The reaction was cooled to room temperature, concentrated in vacuo on a rotary evaporator, and the resultant solids partitioned between ethyl acetate (30 mL) and water (30 mL). The mixture was stirred for 30 minutes, the layers separated, and the aqueous portion extracted with additional ethyl acetate (2×30 mL). The combined organic layers were dried (MgSO4), filtered, and concentrated to a clear oil. The product was purified by passing the oil through a plug of silica gel, eluting with CH2Cl2, 2% methanol in CH2Cl2, 5% methanol in CH2Cl2, and 10% methanol in CH2Cl2. Fractions containing the product were combined and concentrated at reduced pressure to afford the desired product as a light yellow solid. 1.1 g (35%). m.p.: 102–108° C.

1. temperatureThe resulting solution was heated
2. temperatureto reflux for 14 hours, at which time HPLC
3. concentrationconcentrated in vacuo on a rotary evaporator
4. customthe resultant solids partitioned between ethyl acetate (30 mL) and water (30 mL)
5. customthe layers separated
6. extractionthe aqueous portion extracted with additional ethyl acetate (2×30 mL)
7. dry with materialThe combined organic layers were dried (MgSO4)
8. filtrationfiltered
9. concentrationconcentrated to a clear oil
10. customThe product was purified
11. washeluting with CH2Cl2, 2% methanol in CH2Cl2, 5% methanol in CH2Cl2
12. additionFractions containing the product
13. concentrationconcentrated at reduced pressure

ORD record

ord-db4f1558c80e43ddbd6922f876a54dba

Source dataset

uspto-grants-2008_03

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07342003B2