Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Phosphate | HCID 1061 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Product | D-(-)-Fructose | HCID 5984 | — |
Reaction Steps
To 1 of an aqueous solution containing glucose, MgSO4.7H2O and phosphate buffer at a concentration of 0.1 M, 0.005 and 0.05 M, respectively, is added a given amount of insolubilized glucose isomerase and the mixture thus obtained is slowly stirred at a temperature of 70° C. for 60 minutes to effect the conversion. Then, the insolubilized isomerase is separated from the reacted solution and the amount of fructose so produced is determined according to the cystein carbazol method. The activity is calculated as in 3 above.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
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| Patent number | US04205127 |
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| Source dataset | uspto-grants-1980_05 |
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| ORD ID | ord-4d3dd0826a7c4514a5414c0f927bced1 |
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| Source note | uspto-grants-1980_05 |
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