反应详情

温度

55 °C

To 4-chloro-7H-pyrrolo[2,3-d]pyrimidine (9, 5.03 g, 32.8 mmol) in 100 mL of toluene, [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) 1:1 complex with dichloromethane (0.627 g, 0.328 mmol) is added under an atmosphere of nitrogen. After stirring for 10 minutes, methylmagnesium bromide (62.9 mL, 3.00 M in ether, 189 mmol) is added slowly. The reaction is heated at 55° C. overnight, then cooled to −70 to −80° C. and quenched by adding ammonium chloride dropwise. Then 1N hydrochloric acid is added and the pH is adjusted to 7-8 with the addition of saturated sodium bicarbonate. This is extracted 3× with ethyl acetate. The combined organic layer is washed with saturated ammonium chloride and brine, then dried with magnesium sulfate, filtered and the filtrate concentrated under vacuum. The resulting material is purified by silica gel column chromatography, eluting with ethyl acetate and dichloromethane, then methanol and dichloromethane. Appropriate fractions are combined and concentrated under vacuum to provide the desired compound as a tan solid (15). MS (ESI) [M+H+]+=134. This is reacted similarly to steps 2 and 3 of Scheme 4 to provide the desired compound 16.

1. temperaturecooled to −70 to −80° C.
2. customquenched
3. additionby adding ammonium chloride dropwise
4. additionThen 1N hydrochloric acid is added
5. additionthe pH is adjusted to 7-8 with the addition of saturated sodium bicarbonate
6. extractionThis is extracted 3× with ethyl acetate
7. washThe combined organic layer is washed with saturated ammonium chloride and brine
8. dry with materialdried with magnesium sulfate
9. filtrationfiltered
10. concentrationthe filtrate concentrated under vacuum
11. customThe resulting material is purified by silica gel column chromatography
12. washeluting with ethyl acetate and dichloromethane
13. concentrationconcentrated under vacuum