HCID 68751162, compound → HCID 178461962, compound

Participants

Reaction Steps

A mixture of N—((S)-1-(8-chloro-4-fluoro-1-(2-morpholinoethyl)isoquinolin-3-yl)ethyl)-9-(tetrahydro-2H-pyran-2-yl)-9H-purin-6-amine 84 (60 mg, 0.11 mmol) in hydrogen chloride/methanol solution (4 N, 20 mL) was stirred at RT for 0.5 h and then concentrated in vacuo. The residue was suspended in water (20 mL), neutralized with concentrated ammonium hydroxide to adjust the pH to 8-9 and then extracted with DCM (3×10 mL). The combined organic layers were washed with brine, dried over Na2SO4 and filtered. The filtrate was concentrated in vacuo and the residue was purified by flash column chromatography on silica gel (2-10% MeOH-DCM) to afford the product (S)—N-(1-(8-chloro-4-fluoro-1-(2-morpholinoethyl)isoquinolin-3-yl)ethyl)-9H-purin-6-amine 85. ESI-MS m/z: 456.2 [M+H]+.

Provenance & Evidence