1-Methylimidazole → 2-propen-1-yl (3S)-2-((2R)-3-cyclopentyl-2-{[formyl(tetrahydro-2H-pyran-2-yloxy)amino]methyl}propanoyl)-3-({[5-(methyloxy)-2-pyrimidinyl]amino}carbonyl)-1-pyrazolidinecarboxylate

Participants

Reaction Conditions

Reaction Steps

1-Methylimidazole (158 μl, 1.987 mmol) was added into the solution of 2-((2R)-3-cyclopentyl-2-{[formyl(tetrahydro-2H-pyran-2-yloxy)amino]methyl}propanoyl)-1-[(2-propen-1-yloxy)carbonyl]-3-pyrazolidinecarboxylic acid (319 mg, 0.662 mmol) in dichloromethane (DCM) (4.5 ml). The resulting solution was cooled to 0° C., and then mesyl chloride (53.8 μl, 0.696 mmol) was added dropwise at 0° C. To the reaction mixture was added 5-(methyloxy)-2-pyrimidinamine (87 mg, 0.696 mmol). After stirring for 30 min at ambient temperature, the solvent was removed under reduced pressure to give a residue which was purified by RP-HPLC to yield 2-propen-1-yl (3S)-2-((2R)-3-cyclopentyl-2-{[formyl(tetrahydro-2H-pyran-2-yloxy)amino]methyl}propanoyl)-3-({[5-(methyloxy)-2-pyrimidinyl]amino}carbonyl)-1-pyrazolidinecarboxylate (173 mg, 0.294 mmol, 44% yield). MS (ES+) m/z 589.1 (MH+).

Provenance & Evidence