反应详情

温度

5 °C

To a solution of (-)-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid (7.0 g., 0.04 M) and sodium hydroxide (1.6 g., 0.04 M) in water (160 ml.) at 10° C. was slowly added (-)-3-benzoylthio-2-methylpropionyl chloride (9.7 g., 0.04 M). At the same time, from a separate dropping funnel, was added a solution of sodium hydroxide (1.6 g., 0.04 M) in water (15 ml), at such a rate that the solution was maintained at a pH between 9.5 and 7.5 pH units (final pH 7.5). After stirring overnight at 5° C., the reaction mixture was filtered and the clear filtrate acidified with dilute hydrochloric acid. The product was extracted into methylene chichloride, washed and dried over sodium sulfate. After filtration the solvent was removed by evaporation at reduced pressure, leaving a residue (14 g.). This material was separated on an HPLC preparative column (Prep Pak C18 column, Waters) using a mobile phase of 50 parts methanol, 50 parts water and 0.5 parts acetic acid. Two main fractions were obtained, 15% and 84%. The residue from the major fraction was dissolved in ether, washed and dried over sodium sulfate. After filtration the solvent was removed by evaporation at reduced pressure, leaving a white solid, the title compound (10 g.), m.p. 61-63, [α]D25 =-76.39 (C=1.55% EtOH.

1. customat 10° C.
2. temperatureat such a rate that the solution was maintained at a pH between 9.5 and 7.5 pH units (final pH 7.5)
3. filtrationthe reaction mixture was filtered
4. extractionThe product was extracted into methylene chichloride
5. washwashed
6. dry with materialdried over sodium sulfate
7. filtrationAfter filtration the solvent
8. customwas removed by evaporation at reduced pressure
9. customleaving a residue (14 g.)
10. customThis material was separated on an HPLC preparative column (Prep Pak C18 column, Waters)
11. customTwo main fractions were obtained
12. washwashed
13. dry with materialdried over sodium sulfate
14. filtrationAfter filtration the solvent
15. customwas removed by evaporation at reduced pressure
16. customleaving a white solid