86-08-8 → HCID 88312092, compound

HRID 1415861Notes

Reaction equation

HRID 1415861 reaction equation
[NH2:1][C@H](C(O)=O)CCC(O)=O.[CH3:11][C:12]([C:14]1[CH:19]=[CH:18][CH:17]=[N+:16]([CH:20]2[O:24][CH:23]([CH2:25][O:26][P:27]([O:30][P:31]([O:34][CH2:35][CH:36]3[O:40][CH:39]([N:41]4[C:45]5[N:46]=[CH:47][N:48]=[C:49]([NH2:50])[C:44]=5[N:43]=[CH:42]4)[CH:38]([OH:51])[CH:37]3[OH:52])([OH:33])=[O:32])([OH:29])=[O:28])[CH:22]([OH:53])[CH:21]2[OH:54])[CH:15]=1)=[O:13].N[C@H](C([O-])=O)CCC([O-])=O>>[C:12]([C:14]1[CH:15]=[N:16][CH:17]=[CH:18][CH:19]=1)(=[O:13])[CH3:11].[CH:47]1[N:48]=[C:49]([NH2:50])[C:44]2[N:43]=[CH:42][N:41]([C@@H:39]3[O:40][C@H:36]([CH2:35][O:34][P:31]([O:30][P:27]([O:26][CH2:25][C@H:23]4[O:24][C@@H:20]([N:16]5[CH:15]=[C:14]([C:12]([NH2:1])=[O:13])[CH2:19][CH:18]=[CH:17]5)[C@H:21]([OH:54])[C@@H:22]4[OH:53])([OH:29])=[O:28])([OH:33])=[O:32])[C@@H:37]([OH:52])[C@H:38]3[OH:51])[C:45]=2[N:46]=1

Participants

4

Reactant 3

HCID 201186-08-8HCID 33032L-Glutamic AcidHCID 5460544L-glutamate(2-)

Product 1

HCID 88312092HCID 88312092, compound

Procedure

Glutamic acid was determined in the same manner as in Example 3 except that enzyme reaction was carried at 50° C. for 20 minutes in the presence of 3-acetylpyridine NAD (produced by Sigma Co. Ltd.) as coenzyme for glutamate, dehydrogenase (i.e., 3-acetylpyridine NADH formed by enzyme reaction was used as an indicator). The results had a good linear relationship to the real concentrations of glutamic acid. The measurements could be effected in a very short time (about 30 seconds).

Sources & evidence

ORD record
ord-2e10469a48be465f8c33b26268df6fbf
Source dataset
uspto-grants-1999_06
Patent number
US05909114

Reaction notes

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