反应详情

In 3.0 cc of trifluoroacetic acid was dissolved (S)-4-benzyloxycarbonylaminoacetyl-3-(3-t-butoxycarbonylaminopropyl)-2-oxopiperazine-1-acetic acid t-butyl ester (0.6 g, 1.07 mmol), produced in Reference Example 2. The solution was stirred for 30 minutes at room temperature was concentrated under reduced pressure. In 2.1 cc of DMF was dissolved 6-t-butoxyaminocaproic acid (0.26 g, 1.12 mmol), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (0.22 g, 1.14 mmol) and 1-hydroxybenzotriazole (0.15 g, 1.12 mmol). The solution was stirred for one hour, to which was added 2.1 cc of a DMF solution of the concentrate obtained above, and triethylamine (0.3 cc, 2.14 mmol). The mixture was stirred for 4 hours at room temperature. The reaction mixture was diluted with ethyl acetate, washed with a 5% aqueous solution of potassium hydrogen sulfate and a saturated aqueous solution of sodium hydrogen carbonate. The organic layer was concentrated under reduced pressure purified by silica gel column chromatography (ethyl acetate/methanol/acetic acid=20/10/0.6) to afford 0.42 g (y. 63.3%) of the title compound as a colorless amorphous powdery product.

1. customproduced in Reference Example 2
2. concentrationwas concentrated under reduced pressure
3. stirringThe solution was stirred for one hour, to which
4. stirringThe mixture was stirred for 4 hours at room temperature
5. washwashed with a 5% aqueous solution of potassium hydrogen sulfate
6. concentrationThe organic layer was concentrated under reduced pressure
7. custompurified by silica gel column chromatography (ethyl acetate/methanol/acetic acid=20/10/0.6)