Pyridine → 6-Amidino-1-carbamoylmethyl-2-naphthyl 4-[(2-dimethylaminoethyl)aminoiminomethylamino]benzoate

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Reaction Steps

40 Milliliters of 20% hydrous pyridine was added to 1.5 g of 4-[(2-dimethylaminoethyl)aminoiminomethylamino]benzoic acid.dihydrochloride, 1.03 g of 6-amidino-1-carbamoylmethyl-2-naphthol.hydrochloride, 1.44 g of DCC and 56 mg of DMAP, followed by stirring for 2 hours under cooling with ice and then 24 hours at room temperature. The precipitate was filtered and the filtrate was concentrated under reduced pressure. To the residue was added 15 ml of methanol, and this solution was added dropwise to 400 ml of acetone, followed by stirring for 24 hours under cooling with ice. Then, the precipitate was collected by filtration to obtain a crude product. Then, the product was subjected to silica gel column chromatography using methyl ethyl ketone-water-acetic acid (80:15:5) as an eluent, and the desired fractions were collected and the solvent was distilled off under reduced pressure. To the residue were added 15 ml of methanol and 1.41 ml of concentrated hydrochloric acid, and the solution was added dropwise to 400 ml of acetone, followed by stirring for 24 hours under cooling with ice. Then, the precipitate was collected by filtration to obtain 1.52 g of the desired product.

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