HRID14344

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：CC(=O)O.CC(C)C[C@H](NC(=O)CNC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CO)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](Cc1cnc[nH]1)NC(=O)[C@@H]1CCC(=O)N1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N1CCC[C@H]1C(=O)NCC(N)=O.NCC(=O)N[C@@H](CS)C(=O)O

HCID178476261

未命名化合物

PRODUCTS

生成物

结构：CC(=O)O.CC(C)C[C@H](NC(=O)CNC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CO)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](Cc1cnc[nH]1)NC(=O)[C@@H]1CCC(=O)N1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N1CCC[C@H]1C(=O)NCC(N)=O

HCID178476260

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID962

Water

H2O

PROCEDURE

实验过程

Six mg of the GnRH-Gly-Cys hapten (containing an free SH on one end) is dissolved in 1 ml of H2O. The dissolved hapten is added to the activated KLH and allowed to react for 2 hours at room temperature and then overnight in the refrigerator. The KLH-hapten conjugate is immediately purified by applying the reaction mixture to a desalting column. (Sephadex G-25). The conjugate protein should come off on the void volume (first peak, fractions 4-6) as measure by absorbance at 280 nm. There should be a drop in absorbance after fraction 6 and rise in absorbance in fractions 7 or 8 as the excess hapten comes off. A small amount of excess hapten does not cause problem in a prime dose but could neutralize antibody in a boost dose if it is present in large excess. The GnRH/KLH conjugate may be frozen or lyophilized or used in the present form.

WORKUP

后处理

additioncontaining an free SH on one end)
additionThe dissolved hapten is added to the activated KLH
customto react for 2 hours at room temperature
customovernight
customThe KLH-hapten conjugate is immediately purified
temperatureThe GnRH/KLH conjugate may be frozen

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-2d2981e0a166410dbf5d3f4dd745d44d
来源数据集: uspto-grants-2010_06
DOI: 10.6084/m9.figshare.5104873.v1
专利: US07731939B2
来源说明: uspto-grants-2010_06

反应 SMILES

[CH3:1][CH:2]([CH2:4][C@H:5]([NH:31][C:32]([CH2:34][NH:35][C:36]([C@@H:38]([NH:47][C:48]([C@@H:50]([NH:53][C:54]([C@@H:56]([NH:67][C:68]([C@@H:70]([NH:77][C:78]([C@H:80]1[NH:85][C:83](=[O:84])[CH2:82][CH2:81]1)=[O:79])[CH2:71][C:72]1[NH:76][CH:75]=[N:74][CH:73]=1)=[O:69])[CH2:57][C:58]1[C:62]2[CH:63]=[CH:64][CH:65]=[CH:66][C:61]=2[NH:60][CH:59]=1)=[O:55])[CH2:51][OH:52])=[O:49])[CH2:39][C:40]1[CH:41]=[CH:42][C:43]([OH:46])=[CH:44][CH:45]=1)=[O:37])=[O:33])[C:6]([NH:8][C@H:9]([C:17]([N:19]1[C@H:23]([C:24]([NH:26][CH2:27][C:28]([NH2:30])=[O:29])=[O:25])[CH2:22][CH2:21][CH2:20]1)=[O:18])[CH2:10][CH2:11][CH2:12][NH:13][C:14]([NH2:16])=[NH:15])=[O:7])[CH3:3].[CH3:86][C:87]([OH:89])=[O:88].NCC(N[C@H](C(O)=O)CS)=O>O>[CH3:3][CH:2]([CH2:4][C@H:5]([NH:31][C:32]([CH2:34][NH:35][C:36]([C@@H:38]([NH:47][C:48]([C@@H:50]([NH:53][C:54]([C@@H:56]([NH:67][C:68]([C@@H:70]([NH:77][C:78]([C@H:80]1[NH:85][C:83](=[O:84])[CH2:82][CH2:81]1)=[O:79])[CH2:71][C:72]1[NH:76][CH:75]=[N:74][CH:73]=1)=[O:69])[CH2:57][C:58]1[C:62]2[CH:63]=[CH:64][CH:65]=[CH:66][C:61]=2[NH:60][CH:59]=1)=[O:55])[CH2:51][OH:52])=[O:49])[CH2:39][C:40]1[CH:41]=[CH:42][C:43]([OH:46])=[CH:44][CH:45]=1)=[O:37])=[O:33])[C:6]([NH:8][C@H:9]([C:17]([N:19]1[C@H:23]([C:24]([NH:26][CH2:27][C:28]([NH2:30])=[O:29])=[O:25])[CH2:22][CH2:21][CH2:20]1)=[O:18])[CH2:10][CH2:11][CH2:12][NH:13][C:14]([NH2:16])=[NH:15])=[O:7])[CH3:1].[CH3:86][C:87]([OH:89])=[O:88]