L-Glutamine → L-Glutamine

HRID 1435564Notes

Reaction equation

HRID 1435564 reaction equation
B([O-])([O-])[O-].C(N(CC(O)=O)CC(O)=O)C[N:7](CC(O)=O)CC(O)=O.Cl.[CH3:26][O:27][C:28](=[O:32])[C@H:29]([CH3:31])[NH2:30].[NH2:33][C@H:34]([C:40]([OH:42])=[O:41])[CH2:35][CH2:36][C:37](=[O:39])[NH2:38].N[C@H:44](C(O)=O)[CH2:45][C:46](=[O:48])[NH2:47]>>[NH2:30][C@H:29]([C:28]([NH:33][C@H:34]([C:40]([OH:42])=[O:41])[CH2:35][CH2:36][C:37](=[O:39])[NH2:38])=[O:27])[CH3:31].[CH3:26][O:27][C:28](=[O:32])[C@H:29]([CH3:31])[NH2:30].[NH2:33][C@H:34]([C:40]([OH:42])=[O:41])[CH2:35][CH2:36][C:37](=[O:39])[NH2:38].[NH2:7][C@H:45]([C:46]([NH2:47])=[O:48])[CH3:44]

Participants

9

Reactant 5

HCID 5961L-GlutamineHCID 6049Ethylenediaminetetraacetic AcidHCID 6267(-)-AsparagineHCID 26574BorateHCID 2733257Alanine methyl ester hydrochloride

Product 4

HCID 5961L-GlutamineHCID 82335L-Alanine methyl esterHCID 123935L-Alanyl-L-GlutamineHCID 444939L-Alaninamide

Procedure

This purified enzyme was then added to borate buffer (pH 9.0) containing EDTA, L-alanine methyl ester hydrochloride and L-glutamine (or L-asparagine), mixed to the total volume of 1 mL (for the final concentrations, the amount of enzyme added was 2 units as alanine amide decomposition activity, EDTA was at 10 mM, L-alanine methyl ester hydrochloride was at 100 mM and L-glutamine (or L-asparagine) was at 200 mM borate buffer at 100 mM), and allowed to react for 4 hours at 30° C. (Note that the number of units of enzyme does not indicate the production activity with respect to producing L-alanyl-L-glutamine from L-alanine methyl ester and L-glutamine, but rather simply indicates L-alanine amide decomposition activity.) The amount of L-alanyl-L-glutamine produced at this time was 50.2 mM, while the amount of L-alanyl-L-asparagine produced was 49.8 mM.

Workup

  1. additionmixed to the total volume of 1 mL (for the final
  2. concentrationconcentrations
  3. additionthe amount of enzyme added
  4. customto react for 4 hours at 30° C. (

Sources & evidence

ORD record
ord-0f83f64a25ef4536b61a8d8d1500cdaf
Source dataset
uspto-grants-2009_11
Patent number
US07618796B2

Reaction notes

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