反应详情

This purified enzyme was then added to borate buffer (pH 9.0) containing EDTA, L-alanine methyl ester hydrochloride and L-glutamine (or L-asparagine), mixed to the total volume of 1 mL (for the final concentrations, the amount of enzyme added was 2 units as alanine amide decomposition activity, EDTA was at 10 mM, L-alanine methyl ester hydrochloride was at 100 mM and L-glutamine (or L-asparagine) was at 200 mM borate buffer at 100 mM), and allowed to react for 4 hours at 30° C. (Note that the number of units of enzyme does not indicate the production activity with respect to producing L-alanyl-L-glutamine from L-alanine methyl ester and L-glutamine, but rather simply indicates L-alanine amide decomposition activity.) The amount of L-alanyl-L-glutamine produced at this time was 50.2 mM, while the amount of L-alanyl-L-asparagine produced was 49.8 mM.

1. additionmixed to the total volume of 1 mL (for the final
2. concentrationconcentrations
3. additionthe amount of enzyme added
4. customto react for 4 hours at 30° C. (