Sodium Carbonate → 2-Nitrophenol
Reaction equation
[CH:1]1[CH:6]=[C:5]([N+:7]([O-:9])=[O:8])[C:4]([O:10]C2OC(CO)C(O)C(O)C2O)=[CH:3][CH:2]=1.C([O-])([O-])=O.[Na+].[Na+]>>[N+:7]([C:5]1[CH:6]=[CH:1][CH:2]=[CH:3][C:4]=1[OH:10])([O-:9])=[O:8]Participants
3
Reactant 2
Product 1
Procedure
β-Galactosidase Assay. An aliquot of the 14,000 g supernatant from the lysed transfected cells (15-25 μg protein) was incubated at 28° C. for 15-60 minutes with 0.67 mg/ml o-nitrophenyl-β-D-galactopyranoside in a final volume of 1.2 ml (Lee et al., 1984). Reactions were stopped with 0.5 ml 1M Na2CO3, and the amount of o-nitrophenol formed was measured spectrophotometrically at 420 nm.
Sources & evidence
- ORD record
- ord-9e934d51d0aa4b108d12b19dfda3441a
- Source dataset
- uspto-grants-1996_03
- Patent number
- US05498696
Reaction notes
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