Hydrochloric Acid → HCID 59025479, compound

Participants

Reaction Steps

12 mg of the compound from Example 31A (0.019 mol) are dissolved in 2.0 ml of THF/methanol (1:1), mixed with 100 μl of 1 N sodium hydroxide solution and stirred at room temperature for 16 h. The reaction solution is acidified with 1 N hydrochloric acid, diluted with water and extracted three times with dichloromethane. The combined organic phases are dried over sodium sulphate, and the residue is purified by preparative HPLC (eluent: acetonitrile/water with 0.1% formic acid, gradient 20:80→95:5). 6 mg (58% of theory) of the title compound are obtained.

Provenance & Evidence