反应详情

温度

30 °C

A liquid medium (pH 7.0) was prepared so as to contain 4% glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4.7H2O, 0.006% of ZnSO4.7H2O, 0.009% of FeSO4.7H2O, 0.0005% of CuSO4.5H2O, and 0.001% of MnSO4.4 to 5 H2O. The liquid medium was dispensed to large test tubes in an amount of 5 ml per each tube and the test tubes were sterilized by steam at 120° C. for 20 minutes. The liquid medium was inoculated each of the microorganisms shown in Table 3 by a platinum loop and incubated with shaking at 30° C. for 2 to 3 days. Microbial cells were centrifugally collected from each culture solution, and washed with water. After ice cold acetone was added, the cells were dried under reduced pressure to obtain acetone dried microbial cells. 5 mg of the acetone dried microbial cells, 2 mg of ethyl 2-formyl-3-phenylpropionate, 10 mg of glucose, 1 mg of NAD (or NADP), 0.5 ml of 0.1M phosphate buffer (pH=6.5) and 0.5 ml of ethyl acetate were added to each test tube with a cap and shaken at 30° C. for 24 ours. Subsequently, the same operation as in Example 9 was performed to determine a conversion rate and optical purity. The results are shown in Table 3.

1. customA liquid medium (pH 7.0) was prepared so as
2. extractionextract
3. customThe liquid medium was dispensed to large test tubes in an amount of 5 ml per each tube
4. customMicrobial cells were centrifugally collected from each culture solution
5. washwashed with water
6. additionAfter ice cold acetone was added
7. customthe cells were dried under reduced pressure
8. customto obtain acetone dried microbial cells
9. customa cap
10. stirringshaken at 30° C. for 24 ours