Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Sodium Chloride | HCID 5234 | — |
| Reactant | NADP nicotinamide-adenine-dinucleotide phosphate | HCID 5886 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | Nadh | HCID 439153 | — |
| Reactant | Ethyl 2-[(2H-1,3-benzodioxol-5-yl)methyl]-3-oxopropanoate | HCID 10060648 | — |
| Product | HCID 10444903 | HCID 10444903 | — |
Reaction Steps
Each type of the Recombinant Escherichia coli shown in Table 6 was inoculated in 50 ml of 2×YT medium (containing 1.6% of tripeptone, 1.0% of yeast extract, and 0.5% of NaCl, pH=7.0) sterilized in a 500-ml Sakaguchi Flask, and incubated while shaking at 37° C. for 18 hours. To 1 ml of the resultant culture solution, 10 mg of ethyl 2-formyl-3-(3,4-methylenedioxyphenyl)-propionate, 1 mg of NAD (or NADP), and 10 mg of glucose were added and the mixture was stirred at 30° C. for 2 hours. After completion of the reaction, the conversion rate and optical purity of the product were analyzed in the same manner as in Example 9. The results are shown in Table 6 (The conversion rates are all 100%).
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US07666659B2 |
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| Source dataset | uspto-grants-2010_02 |
|---|
| ORD ID | ord-db46348e94484ac9adb54711c87259ec |
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| Source note | uspto-grants-2010_02 |
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