HCID 57863422, compound → HCID 135979853, compound

Participants

Reaction Conditions

Reaction Steps

Dissolve 1-(5-tert-butyl-isoxazol-3-yl)-3-{4-[7-(6-methoxy-pyridin-3-yl)-imidazo[1,2-a]pyridin-3-yl]-benzyl}-urea (0.408 g, 0.82 mmol, 1.0 eq.) in HBr (˜48%, 4 mL) and stir at 80° C. overnight under nitrogen. Evaporate the reaction mixture under vacuum and purify in four steps: 1) flash SCX, 2) reverse phase HPLC The mixture is purified via reversed phase C18 column HPLC employing a gradient of 5 to 65% acetonitrile vs 0.03% aqueous HCl, 3) flash SCX, and 4) silica gel chromatography using a 0-10% MeOH/DC gradient to give the title compound 0.156 g as a white solid: MS(ES), m/z 483 (M+1).

Provenance & Evidence