反应详情

To 7-tert-butoxycarbonylamino-5-cyclopentyl-5-hydroxy-3-oxo-heptanoic acid methyl ester (1.36 g, 3.8 mmol) from Step 3 above dissolved in MeOH (50 mL) was added a NaOH solution (2.8 M, 2.7 mL, 7.6 mmol). The reaction was stirred at room temperature for 18 hours. The reaction was then quenched with acetic acid (0.44 mL, 7.7 mmol) and concentrated by rotary evaporation to an oil. The oil was dissolved in CH2Cl2 and washed with water. The organic layer was separated, and dried over Na2SO4. The solids were removed by filtration, and the liquid was concentrated to yield the product (1.061 g, 86% yield). MS (ESI): 324 (M−H).

1. concentrationconcentrated by rotary evaporation to an oil
2. dissolutionThe oil was dissolved in CH2Cl2
3. washwashed with water
4. customThe organic layer was separated
5. dry with materialdried over Na2SO4
6. customThe solids were removed by filtration
7. concentrationthe liquid was concentrated