Sodium Hydroxide → HCID 68952640, compound

Participants

Reaction Conditions

Reaction Steps

To a solution of methyl 5-fluoro-2-[({[(7S)-3-hydroxy-5-methyl-4-oxo-7-phenyl-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrazin-2-yl]carbonyl}amino)methyl]benzoate (48 mg, 0.106 mmol) in MeOH (1 mL) was added aqueous 1N NaOH (233 μL, 0.233 mmol). The reaction was stirred at 45° C. overnight. Purification by reverse phase chromatography on a C-18 column using a gradient elution of 95-5% H2O (0.1% TFA)/CH3CN (0.1% TFA) and collection and concentration of the appropriate fractions afforded the title product. ES MS (M+1)=439.

Provenance & Evidence