Hydroxyamine hydrochloride → tert-butyl N-[(2R,3S)-3-[[3-benzamido-5-[(E)-N-hydroxy-C-methylcarbonimidoyl]benzoyl]amino]-4-(3,5-difluorophenyl)-2-hydroxybutyl]-N-[(3-methoxyphenyl)methyl]carbamate

Participants

Reaction Conditions

Reaction Steps

A mixture of tert-butyl 3-methoxybenzyl((2R,3S)-3-(3-acetyl-5-(benzamido)benzamido)-4-(3,5-difluorophenyl)-2-hydroxybutyl)carbamate (20 mg, 0.0282 mmol) and hydroxyamine hydrochloride (0.0564 mmol) in ethanol (0.14 mL) was heated at 80° C. for 2 h. The reaction mixture was purified by reverse phase prep HPLC to give the title compound (10 mg): HPLC retention time: 2.255 min (method A). MS (ESI) (M+H)+ 725.53.

Provenance & Evidence