反应详情

Bromine (1 mL) was added to 100 mL of water and the mixture stirred vigorously at rt until all the bromine had dissolved (15 min.). 9-(2'-(diethylphosphonomethoxy)ethyl)guanine (0.360 g, 1.04 mmol) was then dissolved in 10 ml H2O and treated dropwise with the aqueous bromine solution until the color of Br2 persisted. The reaction mixture was allowed to stand at 0° C. for 1 h and then was concentrated to afford a dark yellow viscous gum. Purification was accomplished by column chromatography on silica gel (MeOH--CH2Cl2) to provide 0.31 g of 8-bromo-9-(2'-(diethylphosphonomethoxy)ethyl)guanine as an orange powder.

1. dissolutionhad dissolved (15 min.)
2. concentrationwas concentrated
3. customto afford a dark yellow viscous gum
4. customPurification