Sodium Bicarbonate → HCID 70505182, compound

Participants

Reaction Conditions

Reaction Steps

In 20 ml of 1/15M phosphoric acid buffer solution (pH 6.4) were dissolved 410 mg of 7-(2-thienylacetamido)-2β-methyl-3-acetoxymethylceph-3-em-4-carboxylic acid and 84 mg of NaHCO3, and 139 mg of 1-methyl-5-mercaptotetrazole was then added to the solution. The reaction solution was stirred at 50° C. for 6 hours, while maintaining it at pH 6.4 by pouring 5% aqueous NaHCO3, and then added on a column of Amberlite XAD-II (produced by Rohm & Haas), followed by washing with water and eluting by the gradient method with 0 to 70% aqueous methanol. The eluted fractions were collected, concentrated under reduced pressure and freeze-dried, thus yielding 148 mg (30%) of sodium 7-(2-thienylacetamido)-2β-methyl-3-(1-methyl-1H-tetrazol-5-yl)thiomethylceph-3-em-4-carboxylate.

Provenance & Evidence