Ammonia → HCID 202195, compound

Participants

Reaction Steps

The salt content in the colistin acetate solution from reverse osmosis is adjusted to approx. 200 mM by adding (NH4)2SO4 and pH is adjusted to approx. 7 with diluted ammonia. The concentration of the colistin acetate solution is 15-20 g/l. The solution is passed through the HIC column, flow 950-1050 ml/min. Elution proceeds with 10 times bed volume. Collection of effluent fractions is made automatically by PLC. Samples are drawn from each fraction and analyzed. Fractions complying with pre-set specifications are pooled, yielding a batch with a purity of 94-98% with regards to polymyxin E1. Concentration of polymyxin E1 and removal of ammonium sulphate is performed by ultra filtration to a concentration of 10-20 g/l.

Provenance & Evidence