Isoflurane → Fluasterone

Participants

Reaction Steps

The mice were anesthetized with Isoflurane, and then were given an intramuscular injection of 0.1 mL solution of ketamine (50 mg/kg), xylazine (10 mg/kg) and atropine (0.1 mg/kg). Approximately 10 minutes after the i.m. injection (after the mice could not turn over when placed on their backs), the mice were treated with 10 μL of a 12.5 mg/mL fluasterone/carbitol solution to give a dose of 5 mg/kg. The mice were anesthetized and on their backs for approximately 30 minutes after application of fluasterone/carbitol solution. One hour after the application of fluasterone/carbitol solution, 2 μg of TPA dissolved in 0.2 mL of acetone was applied to the shaved area on the back of each mouse.

Provenance & Evidence