Piperidine → HCID 131736565, compound

Participants

Reaction Steps

Piperidine (0.4 mL) was added to a solution of 7-[2-(6-chloro-5,7-dimethyl-1H,3H-2,4,7a,8-tetraaza-cyclopenta[a]indene-2-carbonyl)-benzyl]-3-oxa-7,9-diaza-bicyclo[3.3.1]nonane-9-carboxylic acid 9H-fluoren-9-ylmethyl ester (354 mg, 0.44 mmol) in DMF (4 mL). The resulting mixture was stirred at RT for 30 min, then diluted with water (20 mL). The precipitate was filtered off and washed with water (2×). After purification by flash chromatography (silica, DCM/EtOH/28% aqueous ammonia), the parent compound was dissolved in water (8 ml) and the solution was lyophilized to give the title compound as a yellow solid (170 mg, 72%). 1H NMR (300 MHz, DMSO-d6) δ 10.92 (br s, 2H), 10.01 (br s, 1H), 7.95-7.78 (m, 2H), 7.72-7.60 (m, 2H), 5.01 (s, 1H), 4.99 (s, 1H), 4.85 (s, 1H), 4.80 (s, 1H), 4.46 (s, 2H), 4.19-4.04 (m, 4H), 3.97-3.70 (m, 6H), 2.86 (s, 1.5H), 2.82 (s, 1.5H), 2.63 (s, 1.5H), 2.57 (s, 1.5H). HPLC (max plot) 100%, Rt 2.26 min. UPLC/MS: (MS+) 467.3 ([M+H]+), (MS−) 465.4 ([M−H]−).

Provenance & Evidence