反应详情

1-Cyano-7-(4-fluoro-benzoylamino)-4-hydroxy-isoquinoline-3-carboxylic acid ethyl ester (70 mg, 0.2636 mmol) was dissolved/suspended in ethanol (4 mL) and 3-Amino-2,2-dimethyl-propionic acid ethyl ester (115 mg, 0.791 mmol) was added via syringe. The reaction was brought to reflux and maintained for 70 h. The reaction was cooled, concentrated and purified by medium pressure liquid chromatography (15 to 50% ethyl acetate in hexanes) to give the product as a white solid which was used immediately in the following reaction.

1. temperatureto reflux
2. temperaturemaintained for 70 h
3. temperatureThe reaction was cooled
4. concentrationconcentrated
5. custompurified by medium pressure liquid chromatography (15 to 50% ethyl acetate in hexanes)