Reaction Steps
Prepared in 89.0% yield from methyl(2S)-2-(tert-butoxy)-2-[(22S)-17-fluoro-5-{[(2-hydroxy-2-methylpropyl)amino]methyl}-4,22,28-trimethyl-21,27-dioxa-1,7,34-triazahexacyclo[26.2.2.16,9.110,14.02,7.015,20]tetratriaconta-2,4,6(34),8,10(33),11,13,15(20),16,18-decaen-3-yl]acetate following the procedure for (2S)-2-(tert-butoxy)-2-[(22S)-8-chloro-4,17,22,28-tetramethyl-21,27-dioxa-1,7,34-triazahexacyclo[26.2.2.16,9.110,14.02,7.015,20]tetratriaconta-2,4,6(34),8,10(33),11,13,15(20),16,18-decaen-3-yl]acetic acid. 1H NMR (500 MHz, DMSO-d6) δ 8.18-8.11 (m, 2H), 7.98-7.93 (m, 1H), 7.52 (t, J=7.7 Hz, 1H), 7.33 (d, J=7.3 Hz, 1H), 7.25-7.12 (m, 3H), 5.71 (br. s., 1H), 4.62 (br. s., 1H), 4.14-4.02 (m, 2H), 3.92-3.81 (m, 1H), 3.74 (br. s., 1H), 3.56-3.41 (m, 6H), 2.64 (d, J=10.6 Hz, 1H), 2.39 (s, 3H), 1.93 (d, J=12.8 Hz, 1H), 1.81 (d, J=13.2 Hz, 4H), 1.74-1.72 (m, 1H), 1.63 (br. s., 4H), 1.19 (s, 3H), 1.11 (s, 9H), 1.08 (d, J=4.4 Hz, 9H); LCMS (ESI, M+1): 745.4.
