N-Acetyl-D-Glucosamine → N-Acetyl-D-Glucosamine

Participants

Reaction Steps

Stock solutions of 10 mM N-acetyl-D-glucosamine, N-[1,2-13C2]acetyl-D-glucosamine, and N-[1,2-13C2]-acetyl-D [1-13C; 15N]glucosamine in water were prepared. The solutions were combined to produce a mixture of the GlcNAc isotopologs at a 1:2:1 molar ratio, respectively. The mixture was analyzed by direct infusion on a Thermo-Finnigan LTQ-XL mass spectrometer set to zoom scan, with the signal averaged over 20 scans. The isotopic ratios were then adjusted by the iterative addition of the desired isotopes until a near-perfect 1:2:1 peak intensity ratio was observed empirically (FIG. 1b). The isotopic labeling composition sample was lyophilized and stored at −20° C.

Provenance & Evidence