Pyridinium hydrobromide → HCID 71576560, compound

Participants

Reaction Steps

To a stirred suspension of 4-(2-aminoethoxy)-7-fluoro-3-(4-pyrrolidin-1-yl-phenyl)-2H-isoquinolin-1-one hydrochloride [(I), cpd. 7, R=R1=R2=H, R3=4-pyrrolidin-1-yl, R4=H](15 mg, 0.037 mmol) in dry THF (0.3 mL), kept at room temperature, pyridinium hydrobromide perbromide (13 mg, 0.04 mmol) were added. The reaction mixture was stirred for 1 hour, the volatiles were then evaporated in vacuo and the resulting crude was purified through preparative HPLC on a Phenomenex Gemini C18 (21×250 mm, 10 μm) column, using a Waters FractionLynx System equipped with a 2996 PDA detector and ZQ2000 single quadrupole mass spectrometer, with electrospray ionization (positive and negative mode). Mobile phase A was 0.05% NH3/ACN 95/5, and mobile phase B was ACN. Gradient from 5 to 95% B in 25 min, hold 95% B 3 min. Flow rate 20 mL/min.

Provenance & Evidence