HCID 117653647, compound → HCID 89663443, compound

Participants

Reaction Steps

Compound 7 (21.9 mg, 0.0401 mmol) was dissolved in EtOAc (1.0 mL) and 10% Pd/C (4.3 mg, 0.43 mg Pd, 0.0040 mmol, 10 mol % Pd) was added and the reaction vessel purged (×4) with H2 (g). The reaction was stirred under a balloon of H2 (g) for 0.5 h, before being purged (×2) with Ar(g). The reaction mixture was filtered through a pad of celite and rinsed with EtOAc. The filtrate was concentrated in vacuo to give the crude hydroxamic acid which was purified by octadecyl functionalized silica gel column chromatography (0:100→100:0 MeOH/H2O) to afford the title hydroxamic acid (JF-B59) (14.6 mg, 80%). A portion of this (8.2 mg) was further purified, for biological testing, by HPLC (gradient elution, 50:50→95:5 MeOH/H2O over 20 mins at 3 mL/min on a semi-preparative Zorbax Rx-C18 column) to afford analytically pure compound (JF-B59) (6.9 mg, 68% extrapolated yield)

Provenance & Evidence