反应详情

One gram (1.6 mmols) of (Z)-1-(3-amino-4-methoxyphenyl)-2-(3,4,5-trimethoxyphenyl)-ethene-Fmoc-L-alanineamide was dissolved in 10 ml of methanol, and 0.9 ml (1.76 mmols) of an aqueous solution of 2-N sodium hydroxide were added thereto. The mixture was stirred for 3 hours. A saturated aqueous solution of sodium chloride was added thereto, and the resulting mixture was extracted three times with dichloromethane. The extract was dried over anhydrous sodium sulfate, and concentrated to dryness under reduced pressure. The product was purified using a silica-gel plate (mixture of 5-% by volume methanol and dichloromethane) to produce 543 mg of the final compound in a yield of 87.9%.

1. extractionthe resulting mixture was extracted three times with dichloromethane
2. dry with materialThe extract was dried over anhydrous sodium sulfate
3. concentrationconcentrated to dryness under reduced pressure
4. customThe product was purified
5. additiona silica-gel plate (mixture of 5-% by volume methanol and dichloromethane)