Trimethyl Phosphate → HCID 474929, compound

Participants

Reaction Conditions

Reaction Steps

2.7 g (6 mmol) N4 -palmitoyl-2',3'-dideoxycytidine is added gradually to a mixture of 1.09 ml (12 mmol) phosphorylchloride and 20 ml trimethylphosphate that is stirred at 0° C. The batch is stirred for a further 2 hours when cold and then slowly neutralized with 0.5N NaOH during cooling. The precipitate that is formed is centrifuged off, absorbed in chloroform/methanol; 7/3; V/V, filtered over sodium sulphate and freed of solvent in a vacuum. The residue so obtained is crystallized several times from ethanol. When this is done, 2.5 g of N4 -palmitoyl-2',3'-dideoxycytidine-5'-phosphate is obtained and this displays an Rf value of 0.35 on the silica gel plate in the chloroform/methanol; 1/1; V/V system.

Provenance & Evidence