Ethylmorpholine → HCID 67709619, compound

Participants

Reaction Conditions

Reaction Steps

0.7 g (6 mmol) of N-ethylmorpholine and 0.8 g (4 mmol) of DCC are added, at 0° C., to 0.7 g (3 mmol) of 4-amidinocinnamic acid hydrochloride, 1.2 g (3 mmol) of methyl N-(2-aminoethyl)-N-(9-fluorenylmethoxycarbonyl)aminoacetate hydrochloride and 0.4 g (3 mmol) of hydroxybenzotriazole in 20 ml of dimethylformamide. The mixture is stirred at 0° C. for one hour and then at room temperature for 20 h, filtered from the precipitated urea and concentrated. The residue is taken up in ethyl acetate and washed with sodium hydrogen carbonate solution, and the organic phase is dried and concentrated. The product is chromatographed, for purification, on Sephadex LH20 using an homogeneous mixture of butanol/glacial acetic acid/water.

Provenance & Evidence