反应详情

Materials. [2-3H] Mannose (15 Ci/mmole) and [6- 3H]galactose (15 Ci/mmole) were purchased from American Radiolabeled Chemicals, Inc. [4,5-3H]Leucine (50 Ci/mmole) was obtained from ICN, pronase was from Calbiochem, and endo-beta-N-acetylglucosaminidase H (Endo H1) was from Miles Scientific. Concanavalin A-Sepharose 4B, maltitol, amyloglucosidase (from Aspergillus niger), beta-glucosidase (from almonds), alpha-galactosidase (from Aspergillus niger), beta-galactosidase (from bovine liver), alpha-mannosidase (from jack bean), and all p-nitrophenyl-glycoside substrates were purchased from Sigma Chemical Company. Beta-Mannosidase was purified from Aspergillus niger as previously described (51). Glucosidase I and glucosidase II were partially purified from mung bean seedlings (50). [3H]Glucose-labeled Glc3Man9GlcNAc-oligosaccharide was isolated from influenza virus-infected MDCK cells labeled with [6-3H]galactose in the presence of castanospermine (52). Tissue culture materials were obtained from Flow Laboratories. Bio-Gel P-4 and Bio-Gel P-2 were purchased from Bio-Rad Laboratories, and Sephadex LH-20 was purchased from Pharmacia Fine Chemicals. Australine was isolated from Castanospermum australe mother liquors by ion exchange chromatography and TLC (53). Castanospermine was isolated from Castanospermum australe mother liquors by crystallization as reported elsewhere (2, 54). All additional chemicals were analytical grade.

1. customBeta-Mannosidase was purified from Aspergillus niger
2. customwere partially purified from mung bean seedlings (50)
3. customwas isolated from influenza virus-infected MDCK cells