反应详情

Exemplary metabolites include D-xylose, D-xylonic acid, 3-deoxy-D-glycero-pentulosonic acid, 3,4-dihydroxy-D-butanal, 1,2,4-D-butanetriol, 4-hydroxy-D-butanal, and 1,4-butanediol. For example, as shown in FIG. 1, a recombinant microorganism can be metabolically engineered to provide elevated expression or heterologous expression of enzymes useful in the production of 1,4-butanediol. For example, xylose is converted to xylonic acid by a xylose dehydrogenase. 3-Deoxy-D-glycero-pentulosonic acid is produced by xylonate dehydratase from xylonic acid. 3-Deoxy-D-glycero-pentulosonoic acid is further converted by a decarboxylase to 3,4-dihydroxy-D-butanal. 3,4-Dihydroxy-D-butanal is then reduced to 1,2,4-D-butanetriol by a dehydrogenase. 1,2,4-D-butanetriol is further dehydrated into 4-hydroxy-D-butanal by a dehydratase. 4-hydroxy-D-butanal was then reduced to 1,4-butanediol by a dehydrogenase.