Water → HCID 71622430, compound

Participants

Reaction Steps

Trifluoroacetic acid (0.44 g, 3.89 mmol) is added to the tert-butyl 2,2′-({[2-(2-hydroxyethoxy)ethyl]imino}bis[pentane-5,1-diyloxybenzene-4,1-diyl(2-methylindolizine-1,3-diyl)carbonyl(2,4-dioxo-1,4-dihydroquinazoline-7,3(2H)-diyl)])diacetate (0.50 g, 0.39 mmol) in suspension in 4 mL of a DCM/MeOH mixture (1/1). The solution is stirred for 8 h at ambient temperature. The reaction mixture is run into water. The precipitate formed is filtered off, washed with water then with ethanol, and dried under vacuum. Purification by preparative HPLC on Kromasil C18 10 μm reverse phase [A=H2O/(CH3COONH4 0.1 M) 90/10; B=CH3CN/(CH3COONH4 0.1 M) 90/10, gradient A/B: 70/30 to 56/44] gives 25 mg (5%) of a yellow powder.

Provenance & Evidence