反应详情

An enzyme reaction liquid in which theanine enzyme synthesis was executed was diluted suitably and thereafter, HPLC was carried out for the diluted reaction liquid so that amounts of theanine and glutamic acid were determined. A mol conversion ratio from an amount of glutamine (mol/L) of the substrate was calculated using obtained amounts of theanine and glutamic acid (mol/L). TABLE 1 shows the conditions for determination by HPLC.

1. customsynthesis
2. additionwas executed was diluted suitably