Hydrochloric Acid → Hexanamide, 3-amino-N-cyclopropyl-2-hydroxy-, hydrochloride (1:1), (3S)-

To tert-butyl-(3S)-1-(cyclopropylamino)-1-oxohexan-3-ylcarbamate (75 mg, 0.26 mmol) was added 4.0 M HCl in dioxane (6.0 mL). After 1 hour, the reaction mixture was concentrated and dried to give the (3S)-3-amino-N-cyclopropyl-2-hydroxyhexanamide HCl salt as a white solid. To this salt in dichloromethane/N,N-dimethylformamide (10:3, 13 mL) was added (2S,4R)-1-(tert-butoxycarbonyl)-4-(6-ethoxyisoquinolin-1-yloxy)-pyrrolidine-2-carboxylic acid (106 mgs, 0.26 mmol), O (7 azabenzotriazol 1 yl) 1,1,3,3 tetramethyl-uronium hexafluorophosphate (HATU) (119 mg, 0.31 mmol) and diisopropylethylamine (0.15 mL, 0.78 mmol). After 1 hour at room temperature reaction mixture was diluted with ethyl acetate and washed with 1N HCl (2×), sodium bicarbonate (1×), and brine (1×). The ethyl acetate layer was dried over magnesium sulfate, filtered and evaporated to dryness under reduced pressure to give (2S,4R)-1-(tert-butyl-2-((3S)-1-cyclopropylamino)-2-hydroxy-1-oxohexan-3-ylcarbamoyl)-4-(6-ethoxyisoquinolin-1-yloxy)pyrrolidine-1-carboxylate.

1. concentrationthe reaction mixture was concentrated
2. customdried

ORD record

ord-ebb17881adda4ff7903a4627860c48a9

Source dataset

uspto-grants-2012_07

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08211897B2